Molecular biology of cholinergic gene locus: choline acetyltransferase and vesicular acetylcholine transporter
نویسندگان
چکیده
منابع مشابه
A unique gene organization for two cholinergic markers, choline acetyltransferase and a putative vesicular transporter of acetylcholine.
Choline acetyltransferase (ChAT) is the biosynthetic enzyme of acetylcholine. In mammalian tissues, it is encoded by multiple mRNAs with different 5'-ends. This diversity results from the alternative usage of three promoters and from differential splicing events. Here, we show that the first intron of the rat ChAT gene contains an open reading frame that encodes a potential vesicular acetylchol...
متن کاملCholine transporter 1 maintains cholinergic function in choline acetyltransferase haploinsufficiency.
Choline acetyltransferase (ChAT), the enzyme that synthesizes the neurotransmitter acetylcholine (ACh), is thought to be present in kinetic excess in cholinergic neurons. The rate-limiting factor in ACh production is the provision of choline to ChAT. Cholinergic neurons are relatively unique in their expression of the choline transporter 1 (CHT1), which exhibits high-affinity for choline and ca...
متن کاملRegulation of cholinergic activity by the vesicular acetylcholine transporter.
Acetylcholine, the first chemical to be identified as a neurotransmitter, is packed in synaptic vesicles by the activity of VAChT (vesicular acetylcholine transporter). A decrease in VAChT expression has been reported in a number of diseases, and this has consequences for the amount of acetylcholine loaded in synaptic vesicles as well as for neurotransmitter release. Several genetically modifie...
متن کاملExpression of the putative vesicular acetylcholine transporter in rat brain and localization in cholinergic synaptic vesicles.
A cholinergic locus has recently been identified consisting of a unique mammalian genomic arrangement containing the genes for choline acetyltransferase (ChAT) and a putative vesicular acetylcholine transporter (VAChT). Although transcripts for ChAT and VAChT protein have been localized in cholinergic neurons, little is known about the encoded VAChT protein. Here we describe production of highl...
متن کاملPhosphorylation of the rat vesicular acetylcholine transporter.
Metabolic labeling of a mutant PC12 cell line, A123.7, expressing recombinant rat vesicular acetylcholine transporter (VAChT) with radiolabeled inorganic phosphate was used to demonstrate phosphorylation of the transporter on a serine residue. Mutational analysis was used to demonstrate that serine 480, which is located on the COOH-terminal cytoplasmic tail, is the sole phosphorylation site. Ph...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1997
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)44647-7